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. 2011 Jul 19;39(20):8728–8739. doi: 10.1093/nar/gkr562

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© The Author(s) 2011. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure. 2. — Expression and splicing of ERVWE1 and ERVFRDE1 proviruses in trophoblastic and non-placental cells. Splicing strategies of (A) ERVWE1 and (D) ERVFRDE1 are shown together with the positions of primers for splicing-specific PCR (arrows) and the sizes of diagnostic PCR products. The levels of non-spliced (open columns) and spliced (black columns) transcripts of (B) ERVWE1 and (E) ERVFRDE1 in BeWo and HeLa cells were estimated by qRT-PCR and are shown as the average percentage of the POLR2A expression ±SD from three triplicates. The low level of genomic DNA contaminating the RT–PCR product of non-spliced ERVWE1 mRNA in BeWo cells is shown (C). Lane 1, marker of molecular size; lane 2, non-spliced ERVWE1 mRNA RT⁺; lane 3, non-spliced mRNA RT⁻; lane 4, spliced mRNA RT⁺; lane 5, spliced mRNA RT⁻; lane 6, marker of molecular size.