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. 2011 Jul 19;39(20):8728–8739. doi: 10.1093/nar/gkr562

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© The Author(s) 2011. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure. 4. — mRNA splicing of ectopically expressed ERVWE1 in JEG-3 (A) and HeLa (B) cells. The pcDNA3 plasmid DNA alone was used for mock-transfected control. Expression of non-spliced (open bars) and spliced (solid bars) ERVWE1 mRNA from de novo integrated proviral DNA was quantified in two independent cell clones of both JEG-3 and HeLa cells by splice-specific qRT–PCR in two independently repeated experiments. Data are presented as the average percentage of the POLR2A expression ±SD from three parallels.