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. 2011 Jul 15;39(20):8765–8777. doi: 10.1093/nar/gkr587

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© The Author(s) 2011. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — The mus81Δ uls1Δ double mutant arrests at G2–M with aberrant cell morphology during MMS treatment. (A) The mus81Δ uls1Δ double mutant accumulates with the G2–M DNA content after 8-h incubation in MMS. (B) The mus81Δ uls1Δ strain exhibits 3-fold increase of cells with morphology defects in the presence of MMS. (C) Western blotting of Rad53 reveals intact checkpoint activation in tested strains after MMS treatment. Asynchronous cells of indicated strains were exposed to MMS and analyzed by flow cytometry to measure DNA content, by microscopy to score the number of cells with altered morphology and used for protein extraction to detect Rad53.