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. 2011 Aug 8;39(20):e133. doi: 10.1093/nar/gkr628

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© The Author(s) 2011. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Constructs drive the expression of various fluorescent proteins and efficiently knock down endogenous target genes. We tested miRNAs against Glypican1 (miGPC1), Axonin1 (miAX1) and Wnt7a (miWnt7a). Chicken embryos were unilaterally electroporated at HH18 with βactin-EBFP2-miGPC1 (A and B), βactin-RFP-miAX1 (C and D), βactin-hrGFPII-miWnt7a (E and F) or βactin-EBFP2-miLuc and examined for target gene expression at HH25-26. In each case strong fluorescence was detectable on the electroporated side (B, D, F and H). ISH showed downregulation of the appropriate target gene on the electroporated side of the neural tube compared to the control side (arrows in A, C and E). Expression of miLuc did not affect the expression of any of the endogenous genes that we tested (G; GPC1 shown as an example). Scale bar: 100 µm.