Figure 2.

Southern hybridization of total Arabidopsis DNA with the complete 4HPPD cDNA as a probe. Total leaf DNA from Arabidopsis was digested (5 μg per reaction) with: lane 1, BamHI; lane 2, EcoRV; lane 3, HindIII; lane 4, PvuI; lane 5, PvuI and BamHI; lane 6, PvuI and HindIII; and lane 7, EcoRV and HindIII. DNA restriction fragments were separated on a 0.8% agarose gel, transferred to a nylon membrane, and then hybridized with the ³²P-labeled probe corresponding to the complete coding region of the 4HPPD cDNA.