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. 2011 Jul 15;39(20):8703–8711. doi: 10.1093/nar/gkr552

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© The Author(s) 2011. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — NRO analysis of the retrotransposon transcription in the spn-E mutants and in the piwi-knockdown flies. NRO analysis of transcription of the endogenous retrotransposons and reporter genes in the ovaries of z2 line (A) and H95 line (B) bearing spn-E mutations. A schematic representation of the reporter construct is given above the diagram. NRO analysis of the endogenous retrotransposon transcription in the ovaries of si_piwi flies (C). Bars of histograms represent a ratio of genes to be tested to rp49 transcript abundance in the spn-E¹/spn-E^hls3987 or si_piwi flies related to this ratio in spn-E¹/TM3 or w¹¹¹⁸, respectively.