Figure 5.

GRN is involved in lysosomal function. (a) GRN and CTSD increase in expression level in HeLa following sucrose treatment, a known inducer of lysosomes, as measured by realtime qPCR. (b) Expression level of GRN and of CTSD increase in HeLa cells following TFEB over-expression, a transcription factor known to regulate lysosome biogenesis. (c) Immunofluorescence with antibody anti-LAMP1 and anti-LAMP2, used as a lysosomal marker, of transfected HeLa cells over-expressing GRN, or EGFP and in HeLa cells grown in medium collected after GRN, or EGFP over-expression.