Figure 3.

Overexpression of MBNL1 partially reverses splicing abnormalities caused by long CAG repeats in HeLa cells. Control—untransfected cells; +MBNL41 lanes—cell lines bearing long CAG tracts transfected with pEGFP-C1MBNL1 plasmid. The level of transcript expressed from pEGFP-C1MBNL1 was determined by RT–PCR analysis. The experiments were carried out in duplicate and quantitative results are shown as bar diagrams. MBNL1 overexpression was estimated in comparison to GAPDH expression level. The fraction of exon inclusion or exclusion (±SD) was calculated by dividing the signal of RT–PCR band corresponding to the inclusion/exclusion splice product by the total RT–PCR signal representing all splice products. *P = (0.05; 0.001), compared with the cells that were not transfected with pEGFP-C1MBNL1 plasmid.