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. 2011 Jul 23;39(20):8891–8900. doi: 10.1093/nar/gkr568

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© The Author(s) 2011. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — Effect of liposomes on mRNA conformation and mRNA translational activity. (A) CD spectra are shown: black line only, mRNA without liposome; dashed line (opened circle), with POPC/Ch(70/30) and dot-dashed line (closed triangle), with POPC/DOTAP(70/30). Final mRNA and lipid concentrations were 0.77 µM and 1.17 mM, respectively. (B) GFP expression with and without liposomes. Final lipid concentration was 1.17 mM; GFP was expressed for 6 h at 30°C. The excitation and emission peaks of GFP were 395 and 509 nm, respectively.