Table 1.
Summary of liposome surface characteristics and the melting temperature (Tm) of tRNA or mRNA on liposomal membrane
| Liposome | Phase | Surface charge densitya (C/nm2) | Membrane fluidity, 1/P (−) |
Tmb (°C) |
|
|---|---|---|---|---|---|
| tRNA | mRNA | ||||
| tRNA/mRNA | – | – | – | 48 | 38 |
| (+)POPC | ldc | ±0 | 5.58 ± 0.42 | 46 | – |
| (+)POPC/Ch (90/10) | ldc | ±0 | 5.28 ± 0.59 | 50 | – |
| (+)POPC/Ch (70/30) | ld+loc | ±0 | 3.54 ± 0.05 | 38 | 33 |
| (+)POPC/Ch (50/50) | loc | ±0 | 2.08 ± 0.09 | 44 | – |
| (+)POPC/DOTAP (70/30) | ldd | + 0.42 | 5.91 ± 0.27 | 53 | – |
| (+)POPC/DOTAP (50/50) | ldd | + 0.70 | 6.17 ± 0.43 | ND | – |
| (+)DOTAP | ldd | + 1.4 | 6.44 ± 0.54 | 62 | 48 |
aSurface charge density of liposome was calculated assuming unilamellarity, spherical shape, a bilayer thickness of 3.7 nm and a mean head group area of 0.72 nm2 (22). bTm was caluculated according to previou report (8). The methods were also shown in Supplementary Figure S3.
cPhase-state of liposome was shown according to De Almedia’s report (23).
dPhase state of POPC/DOTAP liposome was determined by membrane fluidity, 1/P.