Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2011 Jul 20;39(20):8857–8868. doi: 10.1093/nar/gkr595

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2011. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 2. — Aminoacylation of tRNA^Leu-ΔA₇₆, -ΔA₇₆/C75A, -ΔA₇₆/C75U or -ΔA₇₆/C75G by EcLeuRS.Charging plateaus of EctRNA^Leu-ΔA₇₆ (filled diamond), -ΔA₇₆/C75A (filled square), -ΔA₇₆/C75U (filled triangle) or -ΔA₇₆/C75G (filled inverted triangle) were measured as described in the Materials and methods section. The control (unfilled circle) was performed without tRNA. The charging plateaus were determined using GraphPad Prism software. According to the plateau values (to calculate the amount of tRNA^Leu charged leucine) and total tRNA^Leu used in the reaction, EctRNA^Leu-ΔA₇₆, -ΔA₇₆/C75A, -ΔA₇₆/C75U and -ΔA₇₆/C75G are aminoacylated to 70, 70, 61 and 35%, respectively. Digestion of aminoacyl–EctRNA^Leu-ΔA₇₆ by RNase I digestion (unfilled triangle) and no product accumulation in control excluded the self-aminoacylation of the enzyme.