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(a) Gel electrophoretic mobility shift assay of ligand 2 with (CCUG)6 RNA in the presence of 100 nM tRNA. Control lane 1 (C1): RNA only. Control lane 2 (C2): RNA + MBNL1. (b) Gel electrophoretic mobility shift assay of ligand 3 with (CCUG)6 RNA in the presence of 100 nM tRNA. Control lane 1 (C1): RNA only. Control lane 2 (C2): RNA + MBNL1. (c) Plots illustrating inhibition of MBNL1–(CCUG)6 complex with ligand 2 (green) and 3 (blue).
