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Expression of laminin in human MC incubated for 48 h with stimulatory CIC from an IgAN patient or with serum fractions of similar size from a normal control. Lysed MC (same amount of protein was loaded) or culture supernatants (same volume of supernatant was loaded) were separated using 4–20% gradient sodium dodecyl sulfate–polyacrylamide gel electrophoresis under reducing conditions and the blots were probed with polyclonal anti-laminin antibody specific for beta chains.
