Figure 6.

Preassembled DOR1/signaling molecule complexes in LDCVs. (A) In the protein fractions obtained via sucrose-gradient centrifugation, DOR1, G_αi2, G_β1 and PLCβ2 can be seen in the CGRP-containing vesicle fraction (arrow). (B) Proteins from the LDCV-enriched fraction were immunoprecipitated with DOR1 antibodies or IgG. Co-IP shows that DORs are associated with G_αi2, G_β1 and PLCβ2. Three independent experiments were performed. (C) Cell-surface biotinylation and immunoblotting shows that the levels of DOR1, G_αi2 and G_β1 on the cell surface of cultured mouse DRG neurons are elevated by K⁺-induced depolarization in the presence of extracellular Ca²⁺. ^*P < 0.05 versus control (n = 6).