Fig. 2.
CD4+CD8+ thymocytes express more tyrosinated tubulin than mature SP T cells. Purified immature DP and mature SP T cells were cultured for 2 h in the presence (+) or absence (−) of plate-bound anti-TCR/CD2/CD28 antibodies. Protein lysates were separated by SDS–PAGE (8%) and probed with antibodies to tyrosinated tubulin and Akt (loading control). The results of the western blot are shown and relative band intensities (tyrosinated tubulin/Akt) are indicated.