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. Author manuscript; available in PMC: 2011 Oct 28.
Published in final edited form as: Cancer Res. 2007 Dec 1;67(23):11463–11469. doi: 10.1158/0008-5472.CAN-07-2976

Figure 1.

Figure 1

Inhibition of MEK kinase by PD0325901 causes G1 arrest in cells with V600E BRAF mutations. A, IC50 (at 5 d) of cell lines with and without BRAF mutation. Cell proliferation was estimated using the Alamar Blue assay. B, growth kinetics of SKMEL-28 (V600E BRAF) cells treated with PD0325901. Cells were treated with 0 to 100 nmol/L of PD0325901 at the designated concentrations for 1 to 4 d and cell number was estimated using a Coulter counter. Bars, SE. C and D, FACS analysis of V600E BRAF mutant and BRAF WT cells showing that cells with mutant BRAF treated with PD0325901 accumulate in G1. Cells were treated with 25 nmol/L PD0325901 for 48 h. D, apoptosis was not observed in SKMEL-28 cells treated with PD0325901 (25 nmol/L).