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. Author manuscript; available in PMC: 2011 Oct 28.
Published in final edited form as: Cancer Res. 2007 Dec 1;67(23):11463–11469. doi: 10.1158/0008-5472.CAN-07-2976

Figure 2.

Figure 2

MEK inhibition causes a decrease in thymidine uptake in SKMEL-28 (V600E BRAF) cells. A, SKMEL-28 and BT-474 cells were treated with PD0325901 for 48 h and thymidine uptake was measured. Thymidine uptake was inhibited by >90% in SKMEL-28 (V600E BRAF) cells but minimally affected by PD0325901 treatment in BT-474 cells. B, on washout of drug, thymidine uptake was restored to pretreatment levels in SKMEL-28 cells. C and D, treatment of SKMEL-28 cells with PD0325901 (25 nmol/L) resulted in an increase in the fraction of cells staining positive for SA-β-Gal. The photomicrograph in D shows representative fields from control and PD0325901-treated cells at the 48-h time point.