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. 2011 Feb 15;21(6):944–956. doi: 10.1038/cr.2011.27

Figure 5.

Figure 5

IL-4 and IL-13 were involved in cisplatin-mediated cytotoxicity and STAT6 phosphorylation in HEI-OC1 cells. (A) Cells were treated with cisplatin for the indicated periods. The total RNA was then isolated by TRIzol and cDNA was synthesized by reverse transcription. IFN-γ, IL-2, IL-4, IL-5, IL-10, IL-12, IL-13, and GAPDH cDNAs were then amplified using specific primer sets. (B) Cells were pretreated with the indicated doses of neutralizing antibodies including anti-IL-4, anti-IL-5, and anti-IL-13 for 30 min, after which they were further maintained in 20 μM cisplatin for 24 h. The cell viability was then measured by MTT assay. *P < 0.05 as determined by one-way ANOVA when compared with cisplatin-only treated cells. (C) Cells were pretreated with the indicated doses of neutralizing antibodies including anti-TNF-α, anti-IL-4, anti-IL-5, and anti-IL-13 for 30 min and then further maintained in 20 μM cisplatin for 8 h. The cell extracts were then subjected to 12% SDS-PAGE, after which they were immunoblotted with antibodies specific for phospho-STAT6 and STAT6.