Figure 7.

Neither IL-4 nor IL-13 signaling was involved in NF-κB activation in cisplatin-treated HEI-OC1 cells. (A) Luciferase activity of the NF-κB-Luc reporter gene constructs. ^*P < 0.05, by one-way ANOVA when compared with the only-cisplatin treated group. (B) Cells were transfected with 100 nM STAT4, STAT6, and control siRNAs, after which they were incubated for 36 h. The cells were then further treated with 20 μM cisplatin for 2 h. Next, cytosolic and nuclear fractions were prepared as described in Materials and Methods. The extracts were then subjected to 12% SDS-PAGE and immunoblotted with antibodies specific for NF-κB p65, IκB, lamin, and β−actin.