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. 2011 Oct 10;108(43):17773–17778. doi: 10.1073/pnas.1110969108

Fig. 2.

Fig. 2.

DNA homologous recombination repair is suppressed in BRCA1 heterozygous clones. (A) Retroviral vector-based GFP reporter construct BABE-HR measuring homologous recombination efficiency. SceGFP, inactive EGFP in which an 18-bp sequence is substituted with an I-SceI recognition site; iGFP, an internal portion of EGFP; arrows, transcription start sites; ψ, a packaging sequence. (B and C) HR repair assay. Multiple single-cell clones established after BABE-HR infection into MCF-10A-derived isogenic clones were transfected with an I-SceI–expressing plasmid, and GFP-positive ratios were determined by flow cytometry. Shown are a graphic representation of the entire results (B) and dot plots and percentage of GFP positivities in representative single-cell clones (C). Horizontal bars in B are averages. (D) Sensitivity of isogenic cell clones to Doxorubicin (Upper, n = 5) and γ-irradiation (Lower, n = 3). Data are shown relative to those of nontreated cells (mean ± SD).