Fig. 3.

Western blot analysis for GalNAc-Ts and RT-qPCR analysis for GalNAc-Ts and FN IIICS domain. WPE cells were transfected with control siRNA or siRNA for T3/T6, and then treated with TGF-β as described in Materials and Methods. Selectivity of the siRNA was evaluated by Western blot using specific mAbs. (I) Representative Western blot for T2, T3 and T6 from triplicate experiments (A) and mean ± SD (B–D) are shown. (II) Results from RT-qPCR for GalNAc-T2, T3, T6, and FN IIICS domain from triplicate experiments are normalized with values for actin and mean ± SD are shown. RQ, relative quantity. n.s.: not significant . *P ≤ 0.05; **P ≤ 0.005; ***P ≤ 0.001.