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. 2011 Oct 10;108(43):17702–17707. doi: 10.1073/pnas.1113524108

Fig. 4.

Fig. 4.

Loss of MCM9 does not alter MCM2-7 or CDT1 levels, however, leads to mild genomic instability and cell-cycle defects under replication stress. (A) Western blots of mutant and WT MEFS showing detergent soluble vs. chromatin bound levels of indicated proteins. (B) Quantification of data in A. (C) Micronucleus levels in erythrocytes. (D) Chromatid breaks in mutant MEFS. (E) Mcm9 mutant MEFs undergo premature senescence. The y-axis values were taken every 3 d upon passage. (F) Mutant MEFs exhibit a delay in cell-cycle entry following APH-induced replication stress. Primary data are in Fig. S3. MEFs were serum-starved to synchronize at G0/G1, serum was then added, then measurements were taken. AW, Mcm9AWO655; XG. Mcm9XG743. The P value is based on t testing. UN, measurements 20 h after serum addition, but untreated with APH; 20 hr APH, same as previous, but with APH treatement; 3hr Rel or 24hr Rel, same as previous, but 3 h or 24 h after APH was removed from media (n = 3). Error bars indicate SD.