Figure 4.

Tissue-specific expression of the AtGSK1 gene. Total RNA (15 μg) isolated from various tissues was size separated onto a 1.2% formaldehyde/agarose gel and transferred onto a nylon membrane. The northern-blot gel was stained with EtBr, and the gel photograph was taken before transfer to examine the loading of the RNA samples. The whole cDNA (Whole) or the specific probe (Specific) of the AtGSK1 cDNA (nucleotide positions 1149–1513) was used as the hybridization probe, respectively. Hybridizations were carried out in a church buffer at 65°C overnight. The blots were washed with 2× SSC once at room temperature and twice with 0.1× SSC, 0.1% SDS at 60°C for 20 min. F, L, R, and S indicate flower, leaf, root, and silique, respectively.