Figure 7.

Complementation of the mck1 mutant by the AtGSK1 gene. The AtGSK1 gene was introduced into mck1 mutant cells, and the transformed cells were examined for the complementation of temperature sensitivity and NaCl sensitivity of the mutants. The transformed cells were grown liquid SC-Ura⁻ medium at 30°C overnight. The overnight cultures were diluted in YPD medium, plated on YPD, and incubated at 37°C for 3 d to examine temperature sensitivity. To examine NaCl sensitivity the cells were plated in a serial dilution on YPD supplemented with 1.0 m NaCl and incubated at 30°C for 3 d. The numbers on the left side indicate the density of yeast cells.