Fig. 4.

Myc augments mycobacteria-activation of IRAK1, MAPK and NFκB. PBMac were transfected with control or Myc siRNA-a as in Fig. 3 A–C for A–E, and with control or Myc siRNA-b as in Fig. 3 D and E for F–J before BCG (M.O.I. = 1) treatment for the indicated time. Cytoplasmic proteins were analyzed by Western blot. (A and F) The protein level of IRAK1 was quantified, normalized with that of Actin, and expressed as fold change over the treatment with control siRNA-a or siRNA-b and mock. (B–D and G–I) The levels of phosphorylated proteins of EKR1/2, p38 kinase and Akt at each time point were normalized with the level of their respective total proteins and expressed as fold induction over their basal levels at 0 h. (E and J) The protein level of IκBα was normalized with that of Actin at each time point and expressed as fold change over its basal level at 0 h. The data are expressed as mean ± SEM from three (A), five (B), four (C), three (D), four (E), four (F), four (G), three (H), three (I), and three (J) independent blood donors. M denotes mock. * denotes P < 0.05 as determined by Student's t test.