Fig. 3.
Lateral and axial resolution. (A) Transmission image of high-resolution USAF test target. Group 9, elements 1–3 are displayed with line widths ranging from 977–775 nm. FOVxy = 10 μm × 30 μm. (B) Intensity line profile across the vertical bars of group 9, element 1. Using the intensity ratios between the peak and trough values, we determine a one-photon lateral resolution of approximately 1.1 μm (FWHM), which corresponds to a two-photon lateral resolution of approximately 0.8 μm (FWHM). (C) A two-photon axial resolution of approximately 10 μm (FWHM of the thin film response) is determined using a RhB thin film scan.