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. Author manuscript; available in PMC: 2011 Nov 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2010 Sep 9;30(11):2242–2248. doi: 10.1161/ATVBAHA.110.210427

Figure 2.

Figure 2

ApoCIII activates NF-kB in 3T3L1 adipocytes. A and B, 3T3L1 adipocytes were incubated with apoCIII at the indicated concentrations for 30 minutes. In some conditions, apoCIII was pretreated with apoCIII-blocking antibody (50 µg/mL for 30 minutes). Blots represent 4 independent experiments using apoCIII from 4 different donors that yielded similar results. Arrows indicate NF-κB p65 located in the nucleus (B). C and D, 3T3L1 adipocytes were pretreated with SN50 (20 µmol/mL for 30 minutes) and then incubated with apoCIII (100 µg/mL for 8 hours) or PBS (control). *P<0.05 vs control and #P<0.05 vs ApoCIII. IB indicates immunoblotting; p, phosphorylated.