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. 2011 Sep 29;4:190. doi: 10.1186/1756-3305-4-190

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Copyright ©2011 Jiang et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Characteristic motifs and phylogenetic analysis of the six TSP proteins. A: The domains of the TSP proteins were predicted by using CD-Search software. Heavy grey is a higher specific hit while bright grey is that they all contain a tetraspanin-like superfamily domain. B: Homologue phylogenetic tree of sequences from S. mansoni and Sj-TSP-#1~6 (in marked frame) created with the use of MEGA4. These sequences could be subdivided into three major monophyletic subfamilies (the CD family, the CD63 family and the Uroplakin family). Accession number of these sequences: cd151-related [Genbank: XP_002582093.1], Tetraspanin-1 (Tspan-1) [Genbank: XP_002577444.1], tetraspanin family protein 16 invertebrate [Genbank: XP_002573925.1], tetraspanin-CD63 receptor [Genbank: XP_002574749.1], tetraspanin D76 [Genbank: XP_002575497.1], transmembrane 4 superfamily member [Genbank: XP_002579012.1], SjTE736 [Genbank: AAC69992.1], SjTSP2 [Genbank: AEG74364.1], Sj25 [Genbank: AAB88626.1], Sj23 [Genbank: AAA29920.1], Sm23 [Genbank: P19331.1], SmTSP1 [Genbank: AAN17278], SmTSP2 [Genbank: AAN17276.1].