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. 2011 Oct 28;6(10):e24850. doi: 10.1371/journal.pone.0024850

Figure 4. TTR Protein binding assay in human plasma.

Figure 4

(A) - TTR was expressed in bacteria as GST fusion protein. After purification GST alone and GST in fusion with TTR (GST-TTR) were incubated with plasma, affinity purified with gluthathione beads and analysed by SDS-PAGE. Purified fusion protein without plasma incubation (GST-TTR), GST alone incubated with plasma (GST+Control Plasma for incubation with plasma from control individuals and GST+FAP Plasma for incubation with plasma from FAP individuals) were also analysed as controls. Assigned protein bands were excised for identification (Table 2). (B) Western blot analysis of fibrinogen after the pull down assay.