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. 2011 Oct 28;6(10):e24850. doi: 10.1371/journal.pone.0024850

Figure 5. Fibrinogen prevents plasma TTR thermal-induced protein aggregation and glycation decreases it chaperone activity.

Figure 5

(A) Western blot analysis of TTR in the plasma from three control and three FAP individuals after aggregation induced by temperature. (B) Western blot analysis of TTR in the plasma from three FAP individuals after aggregation induced by temperature in the absence of fibrinogen, in the presence of glycated fibrinogen and in the presence of non-glycated fibrinogen. (C) Western blot analysis of fibrinogen in the plasma from three control and three FAP individuals, Western blot of sRAGE was used as loading control. (D) Coomassie blue stained gel of α-crystallin and fibrinogen without glycation and glycated with 2.5 and 10 mM of methylglyoxal. Western blot analysis of argpyrimidine modified proteins. (E) Chemically (DTT) induced protein aggregation of insulin and lysozyme (The aggregation of insulin and lysozyme alone, respectively, are used as controls) in the presence of α-crystallin and fibrinogen with and without glycation. Error bars indicate standard deviation.