Table 2. Nucleotide (nt) and amino acid (aa) changes in NS2B-NS3 fragments of plaque-purified Vero- and MRC-5-passaged DNA-derived DEN-4 2A and DEN-4 2AΔ30 virus clones.

Virus	Cell	Passage Number	Virus Gene Segment	Mutation
				Frequency	Plaque-purified Clone Number	Nucleotide Position	Amino Acid Position
DEN-4 2A	Vero	4	NS3	2/10	3, 8	G 5776 C	R 418 T
DEN-4 2A	Vero	10	NS2B	5/10	2, 4, 5, 6, 9	G 4338 C	G 69 R
DEN-4 2A	Vero	10	NS2B	10/10	1, 2, 3, 4, 5, 6, 7, 8, 9, 10	G 4367 T	Q 78 H
DEN-4 2A	Vero	10	NS2B	7/10	1, 3, 4, 7, 8, 9, 10	G 4455 C	G 108 R
DEN-4 2A	Vero	10	NS2B	3/10	2, 5, 6	G 4470 A	A 113 T
DEN-4 2A	Vero	10	NS3	5/10	3, 4, 5, 8, 10	G 5776 C	R 418 T
DEN-4 2AΔ30	Vero	10	NS2B	10/10	1, 2, 3, 4, 5, 6, 7, 8, 9, 10	C 4224 A	P 31 T
DEN-4 2AΔ30	Vero	10	NS2B	10/10	1, 2, 3, 4, 5, 6, 7, 8, 9, 10	G 4295 C	E 54 D
DEN-4 2AΔ30	Vero	10	NS2B	3/10	1, 5, 8	A 4344 C	S 71 R
DEN-4 2AΔ30	Vero	10	NS2B	10/10	1, 2, 3, 4, 5, 6, 7, 8, 9, 10	G 4356 C	E 75 Q
DEN-4 2AΔ30	Vero	10	NS2B	6/10	1, 2, 3, 5, 8, 9	G 4359 A	V 76 M