Figure 1.

Comparison of the deduced amino acid sequences of MDLs encoded by the black cherry MDL1 to MDL5 and almond MDL cDNAs. Amino acid alignment was performed using the Megalign Clustal program (DNASTAR, PAM 250 residue weight table). MDLa denotes the almond MDL cDNA sequence. Gaps introduced to optimize alignments are designated by dashes. Shaded boxes enclose amino acid residues that are identical in at least two sequences. The N termini of the mature MDL1 and MDL4 proteins are marked by the arrow, and conserved Cys residues are indicated by asterisks. The βαβ-fold comprising the putative FAD-binding site is underlined. Accession numbers for the cDNA sequences are: MDL1, X72617 (Cheng and Poulton, 1993); MDL2, AF040078; MDL3, AF013161; MDL4, AF053384; MDL5, AF053386; and almond MDL (MDLa), Y08211 (Suelves and Puigdomènech, 1998).