Table 2. Cross-reactivity of immune sera with AFs.
Cow number | |||||||||||||
322 | 335 | 338 | 348 | 363 | 366 | ||||||||
AF | IC50 * | % | IC50 | % | IC50 | % | IC50 | % | IC50 | % | IC50 | % | % average |
AFB1 | 76.69 | 100 | 28.06 | 100 | 150.80 | 100 | 58.96 | 100 | 66.11 | 100 | 48.41 | 100 | 100 |
AFB2 | 496.10 | 15 | 213.50 | 13 | 990.00 | 15 | 301.50 | 20 | 282.40 | 23 | 393.30 | 12 | 17 |
AFG1 | 339.60 | 23 | 126.70 | 22 | 502.90 | 30 | 253.10 | 23 | 167.40 | 39 | 100.70 | 48 | 31 |
AFG2 | 906.70 | 8 | 480.70 | 6 | 848.70 | 18 | 2611.00 | 2 | 730.50 | 9 | 405.70 | 12 | 9 |
*IC50 is the AF concentration (ng/ml) causing 50% inhibition of binding of the immune serum to the solid phase.