Figure 3. DUX4 protein overexpression induces different FSHD markers.

24 hours after seeding FSHD and control primary myoblasts were transfected with the indicated expression vectors. Total protein extracts were prepared 48 hours after transfection, 30 µg were separated by electrophoresis (12% PAGE-SDS), transferred to a Western blot and immunodetected with the indicated primary antibodies, appropriate secondary antibodies coupled to HRP and the Lumilight kit (Roche). Actin was stained by Ponceau red on the same membrane before immunodetection and was used as the loading control. A densitometry of the immunoreactive bands was performed. Data are normalized to actin levels in each sample.