Figure 7. Efficiency of AOs pLAM2A (−7+18) and pLAM3A (−12+13) in suppressing endogenous DUX4.

Primary FSHD myoblasts transfected with AOs pLAM2A (−7+18) (50 nM) (A) or pLAM3A (−12+13) (150 nM) (B) or the negative control AO mGMCSF3A(−5+20) (nc-AO, 600 nM) were differentiated for 3 days. Total RNA was extracted. RT-PCR was performed as described in Fig. 5B . The RT-PCR products were analysed by electrophoresis on 1% agarose gel. A densitometry of the bands was performed for quantification. Data are normalized to GAPDH levels in each sample. pGEM42: expression vector containing 2 D4Z4 units [7]; pGEM: empty expression vector; RT (+): with reverse transcriptase; (−): without reverse transcriptase. H₂O: RT-PCR was performed with H₂O. GAPDH: internal control.