Skip to main content
. 2011 Oct 28;6(10):e26815. doi: 10.1371/journal.pone.0026815

Figure 4. ΔNp63α contributes to chemoresistance in pancreatic cancer cells.

Figure 4

A, Effect of cisplatin on ΔNp63α in PDAC. Cells were incubated with 10 µg/mL cisplatin for the initial 6 or full 24 hours. Apoptosis was assessed by monitoring PARP cleavage. B–D, Effect of ΔNp63α on cisplatin-induced apoptosis in PANC-1 (B, C) and T3M4 cells (D). Cells were incubated with 5 µg/mL of cisplatin during indicated time periods. Cells were pelleted after 24 h of incubation, proteins isolated and run on SDS-PAGE gel. Apoptosis was assessed by monitoring PARP and caspase-3 cleavage. A representative image of at least three independent experiments is shown. PANC-1 cells were incubated with 10 µg/mL of cisplatin for the first 12 hours or full 48 hours. MTT assay was done as described in materials and methods. Data are the mean ± SE of three independent experiments.