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. 2011 Oct 28;6(10):e26850. doi: 10.1371/journal.pone.0026850

Table 1. Reference Gene and Genes of Interest in the copepod Calanus helgolandicus RT-qPCR assays.

Acronym Gene name Acc. no. Primer Forward (5′-3′) Primer Reverse (5′-3′) Amplicon size E R2
S20 Ribosomal protein S20 HQ270531 CGTAAGACTCCTTGTGGTGAGG GAAGTGATCTGCTTCACGATCTC 113 89% 0.9915
ATUB Alpha tubulin HQ270529 ACAGCTTCTCCACCTTCTTCTC GTTGTTGGCGGCATCCTC 167 94% 0.9997
BTUB Beta tubulin HQ270528 GGATTTCAGCTGACCCACTC GTCTCATCAGTATTTTCCACCAG 205 97% 0.9862
CYP4 Cytochrome P450-4 JF825512 CTGATCACTCCAACTTTTCACTTC CCATTGCAGTCTCACAGATTATG 169 100% 0.959
ALDH2 Aldehyde Dehydrogenase 2 JF825506 GGACAAGGCAGATGTCAACAA ATAGGGTTTGCCATTGTCAAG 181 100% 0.998
ALDH3 Aldehyde Dehydrogenase 3 JF825507 CCTCTTGGTGTTGTCCTGATC CCAACTCTGATGGCTTGATG 117 95% 0.997
ALDH6 Aldehyde Dehydrogenase 6 JF825508 GAGCAGTGCTGCAGCAACAC GGAACATCCAGAGGGGGATC 164 100% 0.989
ALDH7 Aldehyde Dehydrogenase 7 JF825509 CAGGAGTATGTTGACATCTGTGAC GAAGTTGAAGGCGGTGATG 154 100% 0.988
ALDH8 Aldehyde Dehydrogenase 8 JF825510 CTGGAGGAGTTTGCAGTGG GCCAGCCACACCAATAGG 198 100% 0.997
ALDH9 Aldehyde Dehydrogenase 9 JF825511 GGAAAACCAATCTGGGAAGC CAAAGGGTAGTTCCAGGCTC 183 100% 0.988
GST Glutathione S-Transferase JF825513 CAACCCCCAGCACACTGTG GGATAGACACAATCACCCATCC 210 83% 0.992
GSH-S Glutathione Synthase JF825516 GAGAAGGCAAAGGACTATGCTC GGCAACCTTGTGCATCAAC 180 97% 0.998
CAT Catalase JF825517 TGTACATGCAAAGGGAGCTG GGTGTCTGTTTGCCCACTTT 104 100% 0.998
SOD Superoxide Dismutase JF825518 GGAGATCTTGGCAATGTTCAG CAGTAGCCTTGCTCAGTTCATG 166 97% 0.991
CAS Cellular Apoptosis Susceptibility Protein JF825520 CTACAACCACTACCTGTTCGAGT CAGGGACATGATCTGGAACAC 169 100% 0.995
CARP Cell Cycle and Apoptosis Regulatory 1 Protein JF825519 GCCAAGAGTGGGAAGTTTGAC GAACATTTCATTGAACAATTCTGC 126 98% 0.997
IAP Inibitor of Apoptosis Protein JF825521 CAGGATTCTTCTACACAGGCAG CCATTTCTTGTGTTCTCCCC 108 100% 0.988
HSP70 Heat Shock Protein 70 JF825515 CTTCGTTTGGTATCCATGTTGGTA CTCTGTGTCCTGGTAGGCGAC 130 100% 0.997
HSP40 Heat Shock Protein 40 JF825514 GGATTATTATAAAGTGCTGGGG GTCACTAAGTACATCATAGGCCTC 163 100% 0.996

Table 1 shows Pubmed accession numbers, primer sequences, amplicon sizes (base pair), oligo efficiencies (E) and correlation factors (R2) of the reference gene and genes of interest.