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. 2011 Oct 28;6(10):e26760. doi: 10.1371/journal.pone.0026760

Figure 2. Biochemical analysis of ERBB2 mutants.

Figure 2

(A) HEK293 cells were transfected with either wild type (WT) or mutant ERBB2 for 36 hours and analyzed for autophosphorylation and activation of downstream signaling molecules. Untransfected (UT) cells were taken as control for ERBB2 expression. (B) HEK293 cells were transfected with a combination of ERBB2 (WT or mutant) and EGFR (left two panels) or ERBB3 constructs (right two panels) for 36 hours followed by serum starvation for 12 hours. Cells were then stimulated with either EGF (left two panels) or heregulin (right two panlels) for 5 minutes and analyzed for the activation of ERBB2 as well as downstream signaling pathways by western blotting. Untransfected (UT) cells were used as control.