Figure 7. Binding of the purified Lrp and FimZ proteins to P_fimA.

(A) Binding of FimZ to P_fimA(WT). (B) Binding of Lrp and FimZ to P_fimA(WT). (C) Binding of Lrp and FimZ to P_fimA(WT), P_fimA413 (α), P_fimA529 (β), or P_fimA1225 (γ). The white arrowheads indicate super-shifted Lrp-P_fimA-FimZ complexes. To enhance resolution of the super-shifted DNA-protein complexes, the running time of the polyacrylamide gel in panel C was extended from 1 h (used in panels A and B) to 2 h. Schematic diagrams of the wild-type and mutant fimA promoters are shown under the gel.