Figure 8. Effect of cellular Lrp levels on type 1 fimbriae production in S. Typhimurium.

(A) Western blot analysis for Lrp synthesis and mannose-sensitive yeast agglutination assay for the type 1 fimbriation in strain χ9449 grown statically at 37°C for 24 h in LB medium supplemented with various arabinose concentrations. (B) Western blot analysis for Lrp synthesis and mannose-sensitive yeast agglutination assay in the wild-type strain χ3761 grown statically in various combination of MOPS and LB media at 37°C for 24 h. Agglutination was scored as follows: -, no; +/-, very weak; +, weak; and ++, good. (C) Preparation of standard curve for quantification of cellular Lrp levels by western blot analysis of purified Lrp (100, 50, and 25 ng) using anti-Lrp antiserum and densitometry.