Table 1. Bacterial strains and plasmids used in this study.
Strains | Descriptiona [parental strain] | Source |
S. Typhimurium | ||
χ3761 | Wild-type strain UK-1, highly virulent for chicks and mice | [52], [53] |
χ9411 | Δlrp-13 (lrp-deletion mutation) [χ3761] | [34] |
χ9448 | lrp-1281 (ΔPlrp::Ptrc lrp, chromosomal deletion-insertion mutation to drive constitutive expression of Lrp (lrpC)) [χ3761] | [34] |
χ9449 | ΔrelA198::araC PBAD lacI TT ΔaraBAD23 lrp-1281 [χ9509] | [34] |
χ9455 | PfimA::pYA4311 (PfimA-lacZ), Ampr Gmr [χ3761] | This study |
χ9467 | Δlrp-13 PfimA::pYA4311 (PfimA-lacZ), Ampr Gmr [χ9411] | This study |
χ11107 | PfimA413 (α mutation) [χ3761] | This study |
χ11111 | PfimA529 (β mutation) [χ3761] | This study |
χ11115 | PfimA1225 (γ mutation) [χ3761] | This study |
χ11153 | lrp-1281 PfimA529 [χ9448] | This study |
χ11263 | lrp-1281 PfimA::pYA4311 (PfimA-lacZ), Ampr Gmr [χ9448] | This study |
χ11264 | PfimA413::pYA4311 (PfimA413-lacZ), Ampr Gmr [χ11107] | This study |
χ11265 | PfimA529::pYA4311 (PfimA529-lacZ), Ampr Gmr [χ11111] | This study |
χ11266 | PfimA1225::pYA4311 (PfimA1225-lacZ), Ampr Gmr [χ11115] | This study |
χ11267 | lrp-1281 PfimA529::pYA4311 (PfimA529-lacZ), Ampr Gmr [χ11153] | This study |
χ11377 | PfimA1325 (αγ double mutation) [χ11115] | This study |
χ11378 | PfimA1329 (αβ double mutation) [χ11107] | This study |
χ11379 | PfimA2925 (βγ double mutation) [χ11115] | This study |
χ11380 | PfimA395 (αβγ triple mutation) [χ11379] | This study |
E. coli | ||
MGN-617 (χ7213) | thr-1 leuB6 fhuA21 lacY1 glnV44 recA1 ΔasdA4 thi-1 RP4-2-Tc::Mu [λ-pir], Kmr | [54] |
Plasmids | ||
pRE112 | Positive selection suicide vector (R6K ori) for gene replacement, Cmr | [55] |
pSG3 | a suicide vector (R6K ori) for construction of promoter-lacZ fusion into chromosome | [49] |
pWSK29 | a low-copy-number cloning vector (pSC101 ori), Ampr | [47] |
pYA4124 | Derivative of pET SUMO containing His Tag from pET-14b, Kmr | [34] |
pYA4311 | Derivative of pSG3 for insertion of the PfimA-lacZ fusion into the chromosome, Ampr Gmr | This study |
pYA4758 | Derivative of pRE112 for replacement of the PfimA with PfimA413, Cmr | This study |
pYA4759 | Derivative of pRE112 for replacement of the PfimA with PfimA529, Cmr | This study |
pYA4801 | Derivative of pRE112 for replacement of the PfimA with PfimA1225, Cmr | This study |
pYA4865 | Derivative of pWSK29 harboring a recombinant fimZ gene, Ampr | This study |
Ampr, ampicillin resistance; Gmr, gentamicin resistance; Cmr, chloramphenicol resistance; Kmr, kanamycin resistance.