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. 2011 Oct 28;6(10):e26896. doi: 10.1371/journal.pone.0026896

Table 1. Bacterial strains and plasmids used in this study.

Strains Descriptiona [parental strain] Source
S. Typhimurium
χ3761 Wild-type strain UK-1, highly virulent for chicks and mice [52], [53]
χ9411 Δlrp-13 (lrp-deletion mutation) [χ3761] [34]
χ9448 lrp-1281 (ΔPlrp::Ptrc lrp, chromosomal deletion-insertion mutation to drive constitutive expression of Lrp (lrpC)) [χ3761] [34]
χ9449 ΔrelA198::araC PBAD lacI TT ΔaraBAD23 lrp-1281 [χ9509] [34]
χ9455 PfimA::pYA4311 (PfimA-lacZ), Ampr Gmr [χ3761] This study
χ9467 Δlrp-13 PfimA::pYA4311 (PfimA-lacZ), Ampr Gmr [χ9411] This study
χ11107 PfimA413 (α mutation) [χ3761] This study
χ11111 PfimA529 (β mutation) [χ3761] This study
χ11115 PfimA1225 (γ mutation) [χ3761] This study
χ11153 lrp-1281 PfimA529 [χ9448] This study
χ11263 lrp-1281 PfimA::pYA4311 (PfimA-lacZ), Ampr Gmr [χ9448] This study
χ11264 PfimA413::pYA4311 (PfimA413-lacZ), Ampr Gmr [χ11107] This study
χ11265 PfimA529::pYA4311 (PfimA529-lacZ), Ampr Gmr [χ11111] This study
χ11266 PfimA1225::pYA4311 (PfimA1225-lacZ), Ampr Gmr [χ11115] This study
χ11267 lrp-1281 PfimA529::pYA4311 (PfimA529-lacZ), Ampr Gmr [χ11153] This study
χ11377 PfimA1325 (αγ double mutation) [χ11115] This study
χ11378 PfimA1329 (αβ double mutation) [χ11107] This study
χ11379 PfimA2925 (βγ double mutation) [χ11115] This study
χ11380 PfimA395 (αβγ triple mutation) [χ11379] This study
E. coli
MGN-617 (χ7213) thr-1 leuB6 fhuA21 lacY1 glnV44 recA1 ΔasdA4 thi-1 RP4-2-Tc::Mu [λ-pir], Kmr [54]
Plasmids
pRE112 Positive selection suicide vector (R6K ori) for gene replacement, Cmr [55]
pSG3 a suicide vector (R6K ori) for construction of promoter-lacZ fusion into chromosome [49]
pWSK29 a low-copy-number cloning vector (pSC101 ori), Ampr [47]
pYA4124 Derivative of pET SUMO containing His Tag from pET-14b, Kmr [34]
pYA4311 Derivative of pSG3 for insertion of the PfimA-lacZ fusion into the chromosome, Ampr Gmr This study
pYA4758 Derivative of pRE112 for replacement of the PfimA with PfimA413, Cmr This study
pYA4759 Derivative of pRE112 for replacement of the PfimA with PfimA529, Cmr This study
pYA4801 Derivative of pRE112 for replacement of the PfimA with PfimA1225, Cmr This study
pYA4865 Derivative of pWSK29 harboring a recombinant fimZ gene, Ampr This study
a

Ampr, ampicillin resistance; Gmr, gentamicin resistance; Cmr, chloramphenicol resistance; Kmr, kanamycin resistance.