Figure 6. Lowering the culture temperature increases the current amplitude and slows the rate of desensitization of ASIC1a.

CHO cells were co-transfected with wild-type, W287G or Y71G ASIC1a together with GFP. Cells were cultured overnight at 37°C followed by 24 hrs at 37°C or 27°C. All recordings were performed at the same temperature (∼23°C). (A) Representative pH 6-induced current from cells expressing wild-type or mutant ASIC1a. (B & C) Quantification of the effect of 27°C on current amplitude (B) and the rate of desensitization (C) of wild-type ASIC1a. Asterisks indicate significant differences from 37°C (P<0.01, student's t-test).