Fig. 3.

Comparison of liver fibrosis using TNFR1 KO mice. a, b anti-GFP immunohistochemistry (original magnifications, ×200). Both in wild type mouse live (a) and in TNFR1 KO mouse liver (b), the migrations of GFP positive bone marrow cells were confirmed. c–f Sirius red staining of liver sections (original magnifications, ×40). g Statistical analysis of fibrotic area. Scale bars standard deviations. Compared with WT (Control) (c), GFP/WT (d) had marked fibrolysis. KO (Control) (e) had significantly suppressed fibrosis but there was no marked improvement of fibrosis in GFP/KO (f). h Real-time RT-PCR analysis of expression of collagen type 1 alpha-1, indicating the fibrotic activity, showed similar results. i–l Immunohistochemistry of MMP-9 in liver sections (original magnifications, ×200). Liver section of WT (Control) (i) [magnified figure (×400) (j)] had certain degree of expression, but in KO (Control) liver (k) and in GFP/KO liver (l), the expression was significantly low level. m Real-time RT-PCR analyses. n Western blot analyses of MMP-9; both showed suppression in TNFR1 KO mice, but no significant increase in GFP/KO mice was seen, and the suppression remained