Figure 2.

Analysis of potential HIV target cells in uninfected seminal vesicles. A: Immunohistochemistry performed on uninfected seminal vesicle sections before and during culture (day 9) demonstrated the presence of scattered stromal cells staining positive for CD163, CD3, CD4, CCR5, and CXCR4. CD163⁺ cells were also observed in close contact with epithelial cells (arrows). CD8⁺ cells were found primarily at the level of the epithelium. Scale bars = 20 μm. B: Respective percentages of CD68⁺, CD3⁺, CD4⁺, CD8⁺, CCR5⁺, and CXCR4⁺ cells per surface unit before culture were evaluated in seminal vesicle sections from five donors. Results represent the mean number of positive cells ± SEM. C: HIV receptors bearing cells (CD4⁺, CXCR4⁺, and CCR5⁺) were characterized by double labeling with either CD3 (T-lymphocyte marker) or CD163 (macrophage marker). Nuclei labeled with DAPI are shown (blue). Large panel represents a merged image combining all channels. Side panels represent individual channels. Scale bars = 10 μm.