Figure 4.

Whole mount confocal microscopy showing the distribution of DCs in healing corneal epithelia. Whole corneas of CD11c-DTR mice collected 24 hpw were stained with CD11c antibody (Cy3, red) and examined by confocal microscopy for EGFP. Cy3 and EGFP images were merged. The leading edge of migrating epithelium is marked with a white line in low-magnification images (top panel). The middle and bottom panels are under high magnification. Arrowheads mark the same cell at different magnifications. The figures are representative of three corneas per condition from two independent experiments. No CD11c-positive cells can be identified in the DT-injected cornea.