Figure 7.

Increased cellular apoptosis in DC-depleted corneas assessed by TUNEL staining. CD11c-DTR mice were injected with PBS or DT and then subjected to epithelial debridement at 24 hours after injection. The corneas were collected at 3 and 24 hpw or without wounding for 48 hours [NW (48 hpi)] and cryostat sectioned. The sections were processed by TUNEL staining. Photographs show merged images of TUNEL and nuclear staining of DAPI. Arrows indicate the wound edge. The figure is a representative of three corneas per condition from two independent experiments. E, epithelium; (E), epithelium denuded; S, stroma.