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PMN recruitment in the healing corneal epithelium of DT-depleted mouse corneas. Cryostat sections of PBS- or DT-injected mouse corneas were immunostained with NIMP-R14 to detect PMNs. Photographs show merged images of immunofluorescence of phospho-AKT (red) and nuclear staining of DAPI (blue). Arrows mark the leading edge of migration corneal epithelial cells. The figure is representative of three corneas per condition from two independent experiments. E, epithelium; S, stroma.
