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. 2011 Nov;179(5):2254–2265. doi: 10.1016/j.ajpath.2011.07.046

Figure 7.

Figure 7

Ezrin is activated in MDCK-PC and phospho-negative MDCK-S415A cells, but not in MDCK-S415E cells. A: Western blot analysis of phosphorylated ezrin (Phospho-Ezrin) and total ezrin (Ezrin) in MDCK cells. All MDCK cells show similar levels of total ezrin, but phospho-ezrin is higher in MDCK-PC and MDCK-S415A cells than in MDCK-mock and MDCK-S415E cells. MDCK cells were directly solubilized in 1× sample buffer and immunoblotted with anti-ezrin (1:20,000) or anti-phosphothr567–specific ezrin (1:1000) IgG. Actin was used as a control. B: Densitometric analysis indicating that activated ezrin is greatly increased in MDCK-PC and phospho-deficient MDCK-S415A cells, compared with MDCK-mock cells and phosphomimetic MDCK-S415E cells. Activated ezrin was calculated from the ratios of phospho-ezrin/total ezrin and normalized to the ratio obtained for MDCK-mock cells.