Figure 6.

Cervical adipose tissue from patients with LMNA mutations or HIV infection currently treated using protease inhibitors demonstrates altered adipogenic factors and increased UCP1 expression with prelamin A accumulation. Adipose tissue lysates were submitted to Western blot analysis using antibodies directed against SREBP-1, PPARγ, prelamin A, UCP1, or β-actin (used as an index of total protein expression). Representative blots from triplicate experiments are shown, and quantifications are expressed as fold changes versus control values. The accumulation of prelamin A is associated with increased expression of UCP1 in enlarged cervical fat from patients with LMNA mutations or HIV infection treated using a protease inhibitor–based therapeutic regimen. C, control subject fat samples.