Table 2.

Serologic Parameters of MC4R-KO and WT Mice Fed the HFD for 20 Weeks

	WT mice		MC4R-KO mice
Variable	SD	HFD	SD	HFD
Blood glucose (ad lib, mg/dL)	117.3 ± 5.8	133.0 ± 8.3	167.4 ± 10.3⁎	170.1 ± 12.4
HOMA-IR	0.8 ± 0.3	15.5 ± 3.7⁎	17.0 ± 4.0†	21.8 ± 3.7
TG (mg/dL)	84.6 ± 9.8	46.3 ± 1.2⁎	135.8 ± 18.4⁎	92.7 ± 8.1
FFA (mEq/L)	0.27 ± 0.02	0.26 ± 0.01	0.34 ± 0.02	0.51 ± 0.06‡§
TC (mg/dL)	51.6 ± 2.8	189.7 ± 6.4†	143.0 ± 9.5⁎	294.5 ± 9.2‡¶
Adiponectin (μg/mL)	15.4 ± 1.7	19.8 ± 1.7	13.5 ± 1.0	8.2 ± 0.9‡§
Leptin (ng/mL)	1.7 ± 0.3	97.2 ± 8.3†	57.7 ± 6.0†	112.6 ± 9.2¶
IL-6 (pg/mL)	0.84 ± 0.64	2.21 ± 1.68	3.25 ± 0.75	6.80 ± 0.71‡¶
ALT (IU/L)	36.9 ± 1.1	129.9 ± 19.6	191.9 ± 29.2⁎	623.9 ± 50.8‡¶

n = 7–10. Data are expressed as mean ± SE.

ALT, alanine aminotransferase; FFA, free fatty acid; HFD, high-fat diet; HOMA-IR, homeostasis model assessment–insulin resistance; IL-6, interleukin-6; SD, standard diet; TC, total cholesterol; TG, triglyceride; WT, wild-type.

^⁎P < 0.05,

^†P < 0.01 versus WT-SD;

^‡P < 0.01 versus WT-HFD;

^§P < 0.05;

^¶P < 0.01 versus MC4R-SD.